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Terrestrial ecotoxicology

OECD 208

Terrestrial plants: Seedling emergence and seedling growth test

Test design
Test species Terrestrial monocotiledoneas and/or dicotiledoneas
Life stage Seeds
Route of exposure Contact with seeds
Endpoints Seeling emergence; Wet shoot weight; Dry shoot weight; Phytotoxicity
Test duration 14-21 days after emergence of the control seedling
Test unit 400 g equiv. dry soil
Test concentrations 5
Replicates 4
Specimens 10 seeds/test unit
Temperature 22 +/- 10 °C
Light 16h light/8h dark
Substrate Natural soil or quartz sand
Treatment application Spraying onto the soil surface and seeds or even distribution within the soil portion

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