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Terrestrial ecotoxicology

OECD 207

Acute toxicity to earthworms

Test design
Test species Eisenia fetida
Life stage Adults 2-12 months and 300-600 mg
Route of exposure Contact and ingestion
Endpoints Mortality (LC50) and biomass changes
Test duration 14 days
Test unit 500 g equiv. dry soil (750 g wet soil)
Test concentrations 5
Replicates 4
Specimens 10/test unit
Temperature 20 +/- 2 °C
Light Continuous 400-800 lux
Substrate Artificial soil
Treatment application Even distribution within the soil portion

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