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Terrestrial ecotoxicology

OECD 237 or repeated exposure

Bee larvae toxicity

Test design
Test species Apis mellifera
Life stage Less than 1-day old synchronized larvae
Route of exposure Ingestion
Endpoints Larval mortality and adult emergence rate
Test duration 21 days
Test unit 48-well plates
Test concentrations 5
Replicates 16 replicates for each of 3 bee colonies
Specimens 1 larva per replicate
Temperature Between 34 and 35°C
Light Darkness
Substrate None
Treatment application Bee diets containing the test chemical, on days 3, 4, 5 and 6

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