OECD 237 or repeated exposure
Bee larvae toxicity
| Test design | |
|---|---|
| Test species | Apis mellifera |
| Life stage | Less than 1-day old synchronized larvae |
| Route of exposure | Ingestion |
| Endpoints | Larval mortality and adult emergence rate |
| Test duration | 21 days |
| Test unit | 48-well plates |
| Test concentrations | 5 |
| Replicates | 16 replicates for each of 3 bee colonies |
| Specimens | 1 larva per replicate |
| Temperature | Between 34 and 35°C |
| Light | Darkness |
| Substrate | None |
| Treatment application | Bee diets containing the test chemical, on days 3, 4, 5 and 6 |
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