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Terrestrial ecotoxicology

OECD 227

Terrestrial plants: Vegetative vigour test

Test design
Test species Terrestrial Monocotiledoneas and/or Dicotiledoneas
Life stage 2-4 leaves
Route of exposure Contact
Endpoints Plant height; Dry shoot weight; Phytotoxicity
Test unit 400 g equiv. dry soil
Test concentrations 5
Replicates 4
Specimens 2 to 10 plants/pot, depending on the test species
Temperature 22 +/- 10 °C
Light 16h light/8h dark
Substrate Natural soil or quartz sand
Treatment application Spraying

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