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Terrestrial ecotoxicology

OECD 222

Earthworm reproduction test

Test design
Test species Eisenia fetida
Life stage Adults 2-12 months and 250-600 mg
Route of exposure Contact and ingestion
Endpoints Reproductive output (EC50) and biomass changes
Test duration 8 weeks
Test unit 500 g equiv. dry soil (750 g wet soil)
Test concentrations 8
Replicates 4
Specimens 10/test unit
Temperature 20 +/- 2 °C
Light 16h light(400-800 lux)/8h night
Substrate Artificial soil
Treatment application Even distribution within the soil portion

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