OECD 219
Acute toxicity to Chironomid using spiked water
| Test design | |
|---|---|
| Test species | Chironomus sp. |
| Life stage | First instar larvae |
| Route of exposure | Contact and ingestion |
| Endpoints | Emergence; Development rate |
| Test duration | 28 days |
| Test unit | Sediment-Water volume ratio of 1/4 |
| Test concentrations | 5 |
| Replicates | 4 |
| Specimens | 20/test unit |
| Temperature | 20-25°C |
| Light | 16h light/8h dark |
| Substrate | Artificial soil |
| Treatment application | Spiked water |
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