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Terrestrial ecotoxicology

OECD 219

Acute toxicity to Chironomid using spiked water

Test design
Test species Chironomus sp.
Life stage First instar larvae
Route of exposure Contact and ingestion
Endpoints Emergence; Development rate
Test duration 28 days
Test unit Sediment-Water volume ratio of 1/4
Test concentrations 5
Replicates 4
Specimens 20/test unit
Temperature 20-25°C
Light 16h light/8h dark
Substrate Artificial soil
Treatment application Spiked water

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