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Terrestrial ecotoxicology

OECD 213

Acute toxicity to honey bees – Oral exposure

Test design
Test species Apis mellifera
Life stage Adult worker bees (females)
Route of exposure Ingestion
Endpoints Mortality (LC50)
Test duration 48 hours
Test unit 0.5 L plastic cages
Test concentrations 5
Replicates 3
Specimens 25/test unit
Temperature 25 +/- 2 °C
Light Obscurity
Substrate None
Treatment application Dilution/suspension in a 500 g/L sucrose solution provided as food

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