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Terrestrial ecotoxicology

OECD 221

Lemna sp. Growth Inhibition Test

Test design
Test species Lemna sp.
Life stage Young, rapidly growing plants without visible lesions or discoloration
Route of exposure Contact
Endpoints Growth Inhibition (EC50); Phytotoxicity
Test duration 7 days
Test unit 100 mL of dilution water
Test concentrations 5
Replicates 3
Specimens 3-6 colonies for a total of 9-12 fronds
Temperature 24 +/- 2 °C
Light Intensity PAR : 85 – 135 µE.m-2.s-1
Substrate Dilution water: SIS medium
Treatment application Spiked water

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