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Terrestrial ecotoxicology

OECD 211

Daphnia magna, Reproduction Test

Test design
Test species Daphnia magna
Life stage Juveniles < 24h
Route of exposure Contact and ingestion
Endpoints Reproductive output (EC50)
Test duration 3 weeks
Test unit 40 mL of dilution water
Test concentrations 5
Replicates 10
Specimens 1/test unit
Temperature 18-22 +/- 2 °C
Light 16h Light / 8h Obscurity: max 15-20 µE.m-2.s-1
Substrate Dilution water: M4
Treatment application Spiked water

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