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Terrestrial ecotoxicology

OECD 203

Fish, Acute Toxicity Test

Test design
Test species Brachydanio rerio
Life stage 2.5 cm
Route of exposure Contact and ingestion
Endpoints Mortality (LC50)
Test duration 96 hours
Test unit 4 L of dilution water
Test concentrations 5
Replicates 1
Specimens 7/test unit
Temperature 21-25 +/- 2 °C
Light 16h Light <700lux / 8h Dark
Substrate Dilution water: ISO6341
Treatment application Spiked water

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