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Terrestrial ecotoxicology

OECD 220

Enchytraeid reproduction test

Test design  
Test species Enchytraeus albidus
Life stage Adults
Route of exposure Contact and ingestion
Endpoints Reproductive output (EC50)
Test duration 6 weeks
Test unit 20 g equiv. dry soil (30 g wet soil)
Test concentrations 8
Replicates 4
Specimens 10/test unit
Temperature 20 +/- 2 °C
Light 16h light(400-800 lux)/8h dark
Substrate Artificial soil
Treatment application Even distribution within the soil portion
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New GLP inspection

June 12-13, 2014