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Aquatic ecotoxicology

OECD 202

Daphnia magna, Acute immobilisation test

Test design  
Test species Daphnia magna
Life stage Juveniles < 24h
Route of exposure Contact and ingestion
Endpoints Mortality (LC50) in 48h
Test duration 48 hours
Test unit 10 mL of dilution water
Test concentrations 5
Replicates 4
Specimens 5/test unit
Temperature 18-22 +/- 1 °C
Light Obscurity
Substrate Dilution water: M4
Treatment application Spiked water
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